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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Three chemotypes of thyme (Thymus vulgaris L.) essential oil and their main compounds affect differently the IL-6 and TNFα cytokine secretions of BV-2 microglia by modulating the NF-κB and C/EBPβ signalling pathways

Fig. 4

Effects of essential oil pretreatment on mRNA and protein levels of IL-6 and TNFα. BV-2 cells were pretreated with 200-fold diluted essential oils for 24 h then 1 μg/mL LPS was added to the pretreated BV-2 cells for 24 h. DMSO treatment was used as a control of the treated cells. Real-time PCR was performed with SYBR green protocol using IL-6 and TNFα specific primers. β-actin was used as housekeeping gene for the normalization and relative expression of controls was considered as 1. Proinflammatory cytokine secretions were determined using IL-6 or TNFα specific ELISA kits according to the manufacturer’s protocols. a Relative mRNA expressions of IL-6 and TNFα of the essential oil pretreated cells. b IL-6 and TNFα ELISA measurements from the supernatant of the essential oil pretreated BV-2 cells. The bars represent mean values and error bars represent standard deviation (SD) for three independent determinations (n = 3). Real-time PCR and ELISA measurements were carried out in triplicate in each independent experiments. Asterisks indicate p < 0.05 compared to the control. Crosses show p < 0.05 compared to the LPS treatment

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