Fig. 4

Determination of cell migration ability and epithelial–mesenchymal transition–related proteins using Transwell chamber and Western blot analysis. a Microscopic photography of cells penetrating the upper chamber and statistical analysis of migrating cells in different treatment groups (400×). d-g RT-PCR analysis of expression of EMT transcription factors. h-n Western blot analysis of the expression of EMT marker proteins. ^*P < 0.05, ^**P < 0.01 compared with the control group(n = 3)