Fig. 2

Apoptosis induction of matrine in DLBCL cells through caspase-independent pathway. a-b SU-DHL-16 and OCI-LY3 cells were treated by matrine at different concentrations for 48 h and then determined for apoptotic cells by annexin V and PI staining with flow cytometry. c-d Percent of apoptotic cells induced by matrine at various concentrations. e-f SU-DHL-16 and OCI-LY3 cells were treated with different concentrations of matrine for 48 h, followed by western blot. GAPDH was used as a loading control. Full-length blots are presented in Supplementary Figure 2. Analyses in triplicates. (**p < 0.01 compare to 0 mM group)