Fig. 4

SAL suppresses NLRP3 inflammasome activation by enhancing autophagy. a Representative western blot bands of NLRP3 pathway proteins. b Quantitative results of western blot analysis on expressions of NLRP3, ASC, and caspase-1. HUVECs were pretreated with 3-MA and/or SAL, and then exposed to LPS for 24 h. c MTT shows 3-MA decreases cell viability in HUVECs with LPS and SAL. d Representative blots of NLRP3 protein by western blot analysis. e . 3-MA reverses SAL-induced decrease in NLRP3 protein expression. ***P < 0.001 vs control group; ##P < 0.01, ###P < 0.001 vs LPS group; $$P < 0.01 vs LPS + SAL group