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Table 1 Primer sequences used for RT-PCR (5′-3′)

From: A plant-based medicinal food inhibits the growth of human gastric carcinoma by reversing epithelial–mesenchymal transition via the canonical Wnt/β-catenin signaling pathway

Gene

Sequences

β-catenin

Forward: GGTGGACCCCAAGCCTTAGTA

Reverse: AGATGAAGCCCCAGTGCCT

GSK-3β

Forward: CCACCATCCTTATCCCTCCA

Reverse: AGCGGCGTTATTGGTCTGTC

E-cadherin

Forward: CATCGCCTACACCATCGTCA

Reverse: ACTCTCTCGGTCCAGCCCA

N-cadherin

Forward: CGTGGGAATCAGACGGCTA

Reverse: AAGAGGGAGTCATACGGTGGC

MMP-2

Forward: CTCCCCCGATGCTGATACTG

Reverse: GGTGTCACTGTCCGCCAAAT

MMP-9

Forward: CCAAAGACCTGAAAACCTCCAA

Reverse: TGAAGCATCAGCAAAGCCG

Snail

Forward: TTCACCTTCCAGCAGCCCT

Reverse: TTGCCACTGTCCTCATCGG

Bcl-2

Forward: CCCTGGCATCTTCTCCTTCC

Reverse: TCACGACGGTAGCGACGAG

Bax

Forward: CCCGAGAGGTCTTCTTCCG

Reverse: AAGTCCAGTGTCCAGCCCAT

Caspase-3

Forward: CTGACTGGAAAGCCGAAACTC

Reverse: GGACTGGATGAACCACGACC

Caspase-9

Forward: CTTTGATGGAGATGGCACACC

Reverse: GTCTTTCTGCTCACCACCGC

Cyclin D1

Forward: GGAGCAGAAGTGCGAAGAGG

Reverse: TCACCAGAAGCAGTTCCATTTG

GAPDH

Forward: CCTTCCGTGTTCCTACCCC

Reverse: GCCCAAGATGCCCTTCAGT

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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