Fig. 4

Analysis of concentration dependent effects of VSM on the transcript level in RH-30 cells. The expression factors were all determined densitometrically and normalized to the solvent control, which was set to 1. Transcript expression analysis of RH-30 cells after 48 h VSM treatment (1, 10, 25, 50 and 100 μg/ml) by RT-PCR of ß-actin and GAPDH (loading control); pax3-foxo1 (RMA marker); GLDC, AMT, GCSH and DLD (components of the glycine cleavage system); SGPL1, SPHK1 and SPHK2 (sphingolipid metabolism); as well as ezrin, CXCR4, P-cadherin, syndecan-4, prominin and β-catenin (metastasis and tumor signaling marker). (Representative images of three independent experiments, n = 3)