Skip to main content
Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton

Fig. 3

VSM treatment effects on RH-30 cell morphology. a Bright field images of VSM concentration series (1, 10, 25, 50 and 100 μg/ml VSM) treated RH-30 cells after 48 h compared to the solvent control (0.1% DMSO). b The number of vesicular structures in course of VSM treatment were relatively quantified of five bright field microscopical individual images. Mean ± SD, n = 5, *** P < 0.001, ** P < 0.01, * P < 0.05, significantly different compared to vehicle control, unpaired t-test. c Alterations of the cytoskeleton of RH-30 cells after 25 μg/ml VSM treatment for 48 h compared to the DMSO (0.1%) treatment control. F-actin organization was analyzed via confocal microscopy and was also mathematical processed with the software FilaQuant (University of Rostock, Institute of Mathematics, Mathematical Optimization, Rostock, Germany). F-actin fibers were labelled with Phalloidin Alexa 546 (red) and the cell nuclei with Hoechst (blue). F-actin filaments in the processed images are shown as colored lines. Mean ± SD, unpaired t-test, n = 3, ** P < 0.01, * P < 0.05. d Determination of morphological alterations on RH-30 cells with scanning electron microscopy after 48 h treatment with 25 μg/ml VSM extract compared to the vehicle control (0.1% DMSO)

Back to article page