Fig. 5

Effects of the PKA inhibitor H89 on the induced lipolytic effect of AA-24-a in 3 T3-L1 adipocytes. a Glycerol release into the culture medium. b Western blotting of HSL and phospho-HSL (Ser563, Ser565, and Ser660) in adipocytes preincubated with 20 μM H89 for 1 h followed by treatment with 50 μM AA-24-a for 24 h. c–e Quantification of protein band densities. Data are presented as mean ± standard deviation (n = 3 or 4). *P < 0.05, **P < 0.01, versus control cells. ISO: isoprenaline