Skip to main content

Table 3 Anti-leishmanial activity and selectivity Indexes of fractions from the trunk crude extract against promastigotes and amastigotes of L. donovani

From: Bio-guided isolation of anti-leishmanial natural products from Diospyros gracilescens L. (Ebenaceae)

Extract/ fraction

IC50 (Promastigotes) (μg/ml ± SD)

IC50 (Amastigotes) (μg/ml) ± SD

Raw267.4 CC50 (μg/ml ± SD)

SI (Promastigotes)

SI (Amastigotes)

Trunk crude extract

5.84 ± 0.20f

35.69 ± 0.26f

>  100

>  18.56

>  2.80

Hexane fraction (A)

0.79 ± 0.09b

8.06 ± 0.39b

>  100

>  126.74

>  12.40

Dichloromethane fraction (B)

1.63 ± 0.11d

10.97 ± 0.11c

>  100

>  61.16

>  9.11

Ethyl-acetate fraction (C)

2.36 ± 0.06e

16.05 ± 0.12d

>  100

>  42.30

>  6.23

n-butanol fraction (D)

1.11 ± 0.10c

22.08 ± 0.09e

>  100

>  89.60

>  4.53

Water residue (E)

>  100

–

–

–

–

Positive control

0.34 ± 0.22a

0.12 ± 0.11a

/

/

/

  1. Activity data are mean ± Standard deviation (SD) from triplicate experiments; IC50: 50% Inhibitory Concentration; CC50: 50% Cytotoxic Concentration; SI: Selectivity Index. Along each column, IC50 values with the same letter superscripts are not significantly different, Bonferroni test (p > 0.05)