Investigation of the idiosyncratic hepatotoxicity of Polygonum multiflorum Thunb. through metabolomics using GC-MS

Table 2 Hepatic differential metabolites and metabolic pathways in the different groups

No	t/min	Metabolites	m/z	Trend		Metabolic pathways
1	7.014	Propanoic acid	74.08
2	7.417	l-Valine	117.15
3	7.673	l-Alanine	89.09	↓¹⁾	↓²⁾	Amino acid metabolism
4	7.988	Glycine	75.07	↑¹⁾		Amino acid metabolism
5	8.267	Ethanedioic acid	90.03		↑²⁾	Oxalate metabolism
6	8.518	3-Hydroxybutyric acid	104.10		↑²⁾	Energetic metabolism
7	8.577	leucine	265.30
8	8.894	l-Isoleucine	131.17
9	9.964	Urea	60.06
10	10.133	Serine	105.09
11	10.277	Silanamine	80.59
12	10.386	Silanol	90.20
13	10.535	Hexadecane	260.65
14	10.732	L-Threonine	119.12	↑¹⁾		Amino acid metabolism
15	10.982	Glycine	75.07
16	11.143	Butanedioic acid	118.09
17	11.424	Pyrimidine	80.09
18	11.667	2-Butenedioic acid	116.07
19	11.763	Serine	105.09
20	12.168	N, O, O-Tris (trimethylsilyl)-L-threonine	257.40
21	12.846	l-Aspartic acid,	133.10
22	13.541	Aminomalonic acid	119.08
23	13.851	Malic acid	134.09
24	13.968	Fructose benzoyl oxime	176.17
25	14.342	L-Aspartic acid	133.10
26	14.416	L-Proline	115.13
27	14.576	2-Pyrrolidone-5-carboxylic acid	151.10
28	15.892	Glutamine	169.11
29	16.675	D-Ribose	150.13		↑²⁾	Glucose metabolism
30	17.933	Phosphoric acid	98.00		↑²⁾	Amino acid metabolism
31	19.62	D-Mannose	182.17	↑¹⁾		Glucose metabolism
32	19.845	D-Glucose	180.16	↑¹⁾		Glucose metabolism
33	20.062	D-Galactose	180.16
34	21.887	Hexadecanoic acid	256.42
35	22.29	Inositol	180.16
36	22.76	D-Mannitol	182.17
37	22.837	Octadecane	254.49
38	23.898	Oleic acid	282.46
39	24.268	Octadecanoic acid	284.48
40	28.251	Hexadecanoic acid	256.42		↑²⁾	Fatty acid metabolism

Notes: LPS model group (M) vs. negative control group, ¹⁾ p<0.01, lipopolysaccharide (LPS) + PM high dose group (CPHL) vs. LPS model group, ²⁾ p<0.01. ↑-increase, ↓-decrease. Negative control group (C)

ISSN: 2662-7671