Table 2 Antibodies used in the study
Antibodies | Manufacturer | Catalogue No. | Observed MW | Dilution |
|---|---|---|---|---|
Anti-PI3K | Proteintech | 67,071–1-1 g | 110 KDa | 1:10,000 |
Anti-p-Akt | Proteintech | 66,444–1-1 g | 62 KDa | 1:10,000 |
Anti-Akt | Proteintech | 10,176–2-AP | 56 KDa | 1:5000 |
Anti-p-Bad | Cell Signaling Technology | 5284S | 23 KDa | 1:1000 |
Anti-Bad | Proteintech | 10,435–1-AP | 18 KDa | 1:2500 |
Anti-Bcl-2 | Proteintech | 26,593–1-AP | 26 KDa | 1:2500 |
Anti-Bax | Proteintech | 50,599–2-1 g | 26 KDa | 1:10,000 |
Anti-Caspase-3 | Proteintech | 19,677–1-AP | 32 KDa | 1:2000 |
Anti-cleaved-Caspase-3 | Abcam | ab49822 | 17 KDa | 1:500 |
Anti-Caspase-9 | Proteintech | 10,380–1-AP | 47 KDa | 1: 1000 |
Anti-cleaved-Caspase-9 | Affinity Biosciences | AF5240 | 10 KDa | 1: 1000 |
Anti-PARP1 | Proteintech | 13,371–1-AP | 89 KDa | 1:2000 |
Anti-Cyt-c | Proteintech | 12,245–1-AP | 13 KDa | 1:2000 |
Anti-Mfn1 | Proteintech | 13,798–1-AP | 86 KDa | 1:1000 |
Anti-Mfn2 | Proteintech | 12,186–1-AP | 86 KDa | 1:5000 |
Anti-Drp1 | Proteintech | 10,656–1-AP | 27 KDa | 1:4000 |
Anti-PGC1a | Proteintech | 66,369–1-1 g | 100 KDa | 1:5000 |
Anti-Nrf1 | Proteintech | 12,482–1-AP | 67 KDa | 1:2500 |
Anti-Tfam | Proteintech | 22,586–1-AP | 25 KDa | 1:5000 |
Anti-GAPDH | Proteintech | 60,004–1-1 g | 36 KDa | 1:10,000 |