Table 1 Sequence of primers for RT-PCR and long PCR
Target Gene | Primer Sequence | Size (bp) | Tm (°C) |
|---|---|---|---|
Mfn1 | Forward: 5′-GGGAAGACCAAATCGACAGA-3′ | 152 | 57 |
Reverse: 5′-CAAAACAGACAGGCGACAAA-3′ | 57 | ||
Mfn2 | Forward: 5′-GAGAGGCGATTTGAGGAGTG-3′ | 165 | 58 |
Reverse: 5′-CTCTTCCCGCATTTCAAGAC-3′ | 56 | ||
Drp1 | Forward: 5′-GCCCGTGGATGATAAAAGTG-3′ | 215 | 56 |
Reverse: 5′-TGGCGGTCAAGATGTCAATA-3′ | 56 | ||
PGC-1α | Forward: 5′-GGACGAATACCGCAGAGAGT-3′ | 201 | 59 |
Reverse: 5′-CCATCATCCCGCAGATTTAC-3′ | 56 | ||
Nrf1 | Forward: 5′-AAACCGAACACATGGCTACC-3′ | 168 | 58 |
Reverse: 5′-CTGCCGTGGAGTTGAGTATG-3’ | 58 | ||
Tfam | Forward: 5′-TCACCTCAAGGGAAATTGAAG-3′ | 241 | 55 |
Reverse: 5′-CCCAATCCCAATGACAACTC-3’ | 56 | ||
Long Fragment | Forward:5′-AAAATCCCCGCAAACAATGACCACCC-3’ | 13,400 | 72 |
Reverse: 5′-GGCAATTAAGAGTGGGATGGAGCCAA-3’ | 72 | ||
Shrot Fragment | Forward: 5′-CCTCCCATTCATTATCGCCGCCCTGC-3’ | 235 | 60 |
Reverse: 5′-GTCTGGGTCTCCTAGTAGGTCTGGGAA-3’ | 60 | ||
Bax | Forward: 5′-GCGATGAACTGGACAACAAC-3′ | 200 | 57 |
Reverse: 5′-GATCAGCTCGGGCACTTTAG-3’ | 58 | ||
Bcl-2 | Forward: 5′-CGAGTGGGATACTGGAGATGA-3’ | 236 | 58 |
Reverse: 5′- GACGGTAGCGACGAGAGAAG-3’ | 59 | ||
Caspase-3 | Forward: 5′-CCCATCACAATCTCACGGTAT-3’ | 195 | 57 |
Reverse: 5′-GGACGGAAACAGAACGAACA-3’ | 58 | ||
Caspase-9 | Forward: 5′-GCCTCTGCTTTGTCATGGAG-3’ | 181 | 56 |
Reverse: 5′-AGCATGAGGTTCTCCAGCTT-3’ | 56 | ||
PI3K | Forward: 5′-TCACCTCCCTGATTGGCTAC-3’ | 220 | 58 |
Reverse: 5′-CCACGATGGATGACAATGAA-3′ | 55 | ||
Akt | Forward: 5′-CGAGTCCCCACTCAACAACT-3′ | 231 | 59 |
Reverse: 5′-GGTGAACCTGACCGGAAGTC-3’ | 60 | ||
Bad | Forward: 5′-GAGCTGACGTACAGCGTTGA-3’ | 153 | 60.39 |
Forward: 5′-CCTGAGGGCTGTCCAGTAAC-3’ | 59.75 | ||
PARP | Forward: 5′-AAGCCTGGCACTAAGTCGAA-3’ | 164 | 59.31 |
Forward: 5′-ATAGAGTAGGCGGCCTGGAT-3’ | 59.88 | ||
Cyc-c | Forward: 5′-GGACAGCCCCGATTTAAGTA-3’ | 121 | 57 |
Forward: 5′-TCAATAGGTTTGAGGCGACAC-3’ | 58 | ||
GAPDH | Forward: 5′- AGGTCGGTGTGAACGGATTTG −3’ | 20 | 58 |
Reverse: 5′- GGGGTCGTTGATGGCAACA-3’ | 58 |