Fig. 3

Tanshinone IIA (TIIA) + cyclosporine A (CsA) inhibited myocardial cells apoptosis after renal ischemia-reperfusion (IR). Rats were pre-treated with TIIA alone or in combination with CsA followed by removing the right kidney and clamping of the left renal artery for 30 min and reperfusion for 24 h. Sham rats were used as control. Representative apoptosis of myocardial cells (a), TUNEL positive cells (a), the scale bars represents a length of 100 μm on histology, the activity of myocardial caspase-9/3 (b, c), and the protein expression of cleaved caspase-9/3 (d) were evaluated under different groups. Data are shown as mean ± SD. *p < 0.05 versus sham group, ^#p < 0.05 versus IR (obese) group, ^△p < 0.05 versus TIIA group, ^▲p < 0.05 versus CsA group