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Fig. 1 | BMC Complementary Medicine and Therapies

Fig. 1

From: Trichosanthin cooperates with Granzyme B to restrain tumor formation in tongue squamous cell carcinoma

Fig. 1

Effect of TCS on the viability of oral cancer cells in vitro. a. SCC15 and SCC25 cells were cultured in the presence of different concentrations of TCS for 48 h to 72 h. The remaining cell viability was measured by CCK8 assay. b. Cell viability of SCC-25 cells upon treatments of TCS and cisplatin for 72 h at the indicated concentrations. Cell viability was evaluated by MTS assay. Only TCS at higher concentrations displayed statistically significant reductions relative to the lower group (n = 5). c. TCS induced the apoptosis of SCC25 cells in a concentration-dependent manner. TUNEL positive signal cells were captured following treatment with TCS (5, 50, and 350 μg/mL) for 48 h. Z-VAD-FMK (pan-caspase inhibitor, 20 μM) treatment inhibited TCS-induced for 48 h. ABT-263(8 μM) for 48 h was used as the positive control. (TUNEL: green). d. Western blotting analysis to detect Bcl-2 in SCC25 cells after various TCS treatments. Values are presented as the mean ± standard error of the mean from a minimum of three independent experiments. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001

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