Fig. 1From: Trichosanthin cooperates with Granzyme B to restrain tumor formation in tongue squamous cell carcinomaEffect of TCS on the viability of oral cancer cells in vitro. a. SCC15 and SCC25 cells were cultured in the presence of different concentrations of TCS for 48 h to 72 h. The remaining cell viability was measured by CCK8 assay. b. Cell viability of SCC-25 cells upon treatments of TCS and cisplatin for 72 h at the indicated concentrations. Cell viability was evaluated by MTS assay. Only TCS at higher concentrations displayed statistically significant reductions relative to the lower group (n = 5). c. TCS induced the apoptosis of SCC25 cells in a concentration-dependent manner. TUNEL positive signal cells were captured following treatment with TCS (5, 50, and 350 μg/mL) for 48 h. Z-VAD-FMK (pan-caspase inhibitor, 20 μM) treatment inhibited TCS-induced for 48 h. ABT-263(8 μM) for 48 h was used as the positive control. (TUNEL: green). d. Western blotting analysis to detect Bcl-2 in SCC25 cells after various TCS treatments. Values are presented as the mean ± standard error of the mean from a minimum of three independent experiments. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001Back to article page