Fig. 3
Effect of Clitoria ternatea flower extract (CTE) on DNA strand breakage in methylglyoxal (MG)/lysine and 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) system. Supercoiled form (SC) and opened circular form (OC) represented as the major band and damaged plasmid DNA, respectively. Plasmid DNA (0.25 μg) was incubated with the following: a Lane 1, DNA alone; Lane 2, 50 mM MG; Lane 3, 50 mM lysine; Lane 4, 0.25 mg/mL CTE; Lane 5, 1 mg/mL CTE; Lane 6, MG + lysine; Lane 7, MG + lysine+Cu2+; Lane 8, MG + lysine+Cu2++ 0.25 mg/mL CTE; Lane 9, MG + lysine+Cu2++ 0.5 mg/mL CTE; Lane 10, MG + lysine+Cu2++ 1 mg/mL CTE; Lane 11, 12.5 mM AAPH; Lane 12, AAPH+ 0.125 mg/mL CTE; Lane 13, AAPH+ 0.25 mg/mL CTE; Lane 14, AAPH+ 0.5 mg/mL CTE; Lane 15, AAPH+ 1 mg/mL CTE. The percentage of open circular (OC) form of plasmid DNA in b MG/lysine- and c AAPH-mediated oxidative strand breakage of plasmid DNA. Each value represents the mean ± SEM (n = 3). Means with different letters are significant different (p < 0.05)