Fig. 1

Betulinic acid inhibits PANC-1 and SW1990 cells proliferation. CCK8 assay of PANC-1 (a) and SW1990 (c) cells incubated with 5 μM,10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 90 μM. Betulinic acid or an equal volume of DMEM medium for 24 h. Label-free Real-time Cellular Analysis (RTCA) following PANC-1 (b) and SW1990 (d) cells incubated with betulinic acid (20 μM, 60 μM) or an equal volume of DMEM medium for 24 h. (e) Ki67 Immunofluorescence following PANC-1 and SW1990 cells incubated with betulinic acid (20 μM, 60 μM) or an equal volume of DMEM medium for 24 h