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Table 1 PCR protocol for M2 gene amplification

From: Anti-influenza A virus activity of two Newtonia species and the isolated compound myricetin-3-o-rhamnoside

Step

Temperature (°C)

Time

Number of cycles

Initial denaturation

95

10 min

1

Denaturation

95

10 s

35

Annealing

55

10 s

Extension

72

30 s

Final elongation

72

5 min

1