Fig. 3

Flow cytometric analysis of Rhodamine 123 efflux capacity in CSCs after treatment with TQ (a). Treatment with TQ inhibited the Rhodamine 123 efflux capacity and increased accumulated intracellular Rhodamine 123 inside the CSCs (a-b). The percent of CD24⁻ CSCs was also determined using flow cytometry (c). Results showed the increase of CD24⁺ CSCs and stemness loss in condition containing TQ. One-Way ANOVA with Tukey posthoc analysis. ****p < 0.0001