Antiplasmodial activity of Ethanolic extract of Cassia spectabilis DC leaf and its inhibition effect in Heme detoxification

Table 5 Stage-specific activity of 90% ethanolic extracts of C. spectabilis DC leaf against P. falciparum 3D7 strain

Samples	Incubation time (hours)	Parasitic stage			% parasitemia	% growth	% inhibition
Samples	Incubation time (hours)	Ring	Trophozoite	Schizont	% parasitemia	% growth	% inhibition
Negative control	0	41	5	1	0.76	–	–
	6	38	11	2	0.84	0.08	–
	12	36	15	2	0.86	0.10	–
	24	45	19	7	1.14	0.38	–
	48	95	30	4	2.06	1.30	–
90% ethanolic extract of C. spectabilis DC leaf	0	41	5	1	0.76	–	–
	6	38	10	1	0.81	0.05	37.5
	12	34	11	5	0.79	0.03	70
	24	22	13	2	0.59	0	100
	48	17	0	0	0.28	0	100

% parasitemia was obtained from total number of parasites divide to ring, trophozoite and schizont
% growth was obtained from % parasitemia an incubation time minus % parasitemia at 0 h
% inhibition was calculated according to the following formula: % inhibition = ((parasitemia in negative control – parasitemia in treatment group) / parasitemia in negative control) × 100
Negative control: The cultures were only added with DMSO, without any extract. The DMSO was used as negative control because DMSO was used to dissolve the extract

ISSN: 2662-7671