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Fig. 2 | BMC Complementary Medicine and Therapies

Fig. 2

From: Icariin promotes the repair of PC12 cells by inhibiting endoplasmic reticulum stress

Fig. 2

The results of CCK8 assay, SRB assay and flow cytometry assay. A The induced PC12 cells were cultured for 24 h, 48 h and 72 h, and CCK8 assay was carried out. The results showed that the OD value of each group increased gradually. At 72 h, there was no significant difference in OD value between DMSO group and blank group, OD value in TG group was significantly lower than that in DMSO group, and OD value in ICA group was significantly higher than that in TG group. B The induced PC12 cells were cultured for 24 h, 48 h and 72 h, and SRB assay was carried out. The results showed that the OD value of blank group and DMSO group increased gradually, but that of TG group showed a downward trend, and that of ICA group tended to be stable at 48 h. At 72 h, there was no significant difference in OD value between DMSO group and blank group, OD value in TG group was significantly lower than that in DMSO group, and OD value in ICA group was significantly higher than that in TG group. C The induced neurons were cultured for 48 h and double stained by AnnexinV-FITC and PI. The results showed that there was no significant difference in the apoptosis rate between the DMSO group and the blank group, the apoptosis rate in the TG group was significantly higher than that in the DMSO group, and the apoptosis rate in the ICA group was significantly lower than that in the TG group

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