Fig. 5

The effects of EP and CS extracts on cellular ROS level in human lung cancer cells. The A549 cells were treated with 100 and 300 ng/mL of EP and CS extracts, separately, for 36 h and subjected to ROS measurement. A: represents the flow cytometry histograms indicating the un-treated cells (a), cells treated with 100 ng/mL of EP (b), 300 ng/mL of EP (c), 300 ng/mL of EP + 50 ng/mL of AM630 (d), cells treated with 100 ng/mL of CS (e), 300 ng/mL of CS (f), and 300 ng/mL of CS + 50 ng/mL of AM630 (g). B: represents the mean level of ROS following EP treatment. C: represents the mean level of ROS following CS treatment. Data represent mean ± SD of three separate experiments. The statistical differences between treated and untreated groups were analyzed by ANOVA and are indicated by asterisks (*** = P < 0.001)