Evaluation of antimicrobial activity of the hydrolate of Coridothymus capitatus (L.) Reichenb. fil. (Lamiaceae) alone and in combination with antimicrobial agents

Table 5 Fractional inhibitory concentration (FIC) and FIC indices (FICI) of ITC-hydrolate of C. capitatus pairs against Candida strains

Yeasts		MICa	MICc	FIC	FICI	Type of interaction
C. krusei 398	Itraconazole	2	0.25	0.125	0.375	Synergy
C. krusei 398	Hydrolate	25 (0.035)^a	6.25 (0.009)^a	0.25
C. albicans 183	Itraconazole	0.5	0.06	0.12	0.62	Additive
C. albicans 183	Hydrolate	50 (0.070)	25 (0.035)	0.5
C. albicans ATCC 90028	Itraconazole	0.5	0.125	0.5	1	Additive
C. albicans ATCC 90028	Hydrolate	50 (0.070)	25 (0.035)	0.5
C. albicans ATCC 10231	Itraconazole	1	1	1	2	Indifference
C. albicans ATCC 10231	Hydrolate	50 (0.070)	50 (0.070)	1
C. glabrata 32–09	Itraconazole	1	1	1	2	Indifference
C. glabrata 32–09	Hydrolate	6.25 (0.009)	6.25 (0.009)	1

(As essential oil % (v/v) content in hydrolate product)^a
MICa, MIC of one sample alone; MICc, MIC of one sample of the most effective combination
FIC of itraconazole = MIC of itraconazole in combination with hydrolate/MIC of itraconazole alone
FIC of hydrolate = MIC of hydrolate in combination with itraconazole/MIC of hydrolate alone
FICI = FIC of itraconazole+FIC of hydrolate
FICI ≤0.5, synergy; FICI > 0.5 and ≤ 1, additive; FICI > 1 and ≤ 4, indifference; FICI > 4 antagonism

ISSN: 2662-7671