Fig. 5
Overexpressing miR-124 attenuated LPS/D-GalN-induced acute liver injury and the activity of the Hedgehog signaling pathway. a Representative images of HE-stained liver sections from four experimental groups (magnification, × 400) after the administration of 1 × 109 pfu AdCMV-miR-124 for 14 d and intraperitoneal administration of LPS and D-GalN to induce acute liver injury. b and c Representative RT-PCR showing the miR-124 expression levels in LPS and D-GalN to induce liver injury 14 d after the administration of 1 × 109 pfu AdCMV-miR-124 and intraperitoneal administration of LPS and D-GalN to induce acute liver injury. d and e Representative Western blot analysis and statistical summary of densitometric analysis showing Hedgehog, Ptch, Smo, Gli, p-IkB-α, and NF-kB65 protein expression levels in liver tissue 24 h after the administration of 1 × 109 pfu AdCMV-miR-124 for 14 d and intraperitoneal administration of LPS and D-GalN to induce acute liver injury. f Liver injury score from four experimental groups (magnification, × 400) after the administration of 1 × 109 pfu AdCMV-miR-124 for 14 d and intraperitoneal administration of LPS and D-GalN to induce acute liver injury. Data are expressed as mean ± SD. *P < 0.05: compared with the control group; #P < 0.05 and ##P < 0.01 compared with the model group