Fig. 5

Germacrone alleviated OGD/R-induced PC12 cells injury by inhibiting autophagy (A) Detection of the LDH release in each group by kit assay (n = 6). (B) Detection of the cell viability of each group by MTT assay (n = 6). (C) Detection of the expression of LC3 in cells of each group by Western Blot (n = 3). (D) Observation of the ultrastructural of PC12 cells without OGD/R injury(a) and after OGD/R 24 h injury(b), as well as the ultrastructural of PC12 cells treated with 20 μM (c), 40 μM (d), 80 μM(e) of germacrone and 3-MA(f) after OGD/R 24 h injury by TEM (× 20,000). Note: ^##P < 0.01, vs control group; ^*P < 0.05, ^**P < 0.01, vs OGD/R group