Fig. 5

Soyasaponins inhibit the protein expression of MyD88 and TRAF6, and activation of NF-κB in MyD88-transfected HEK293T cells. HEK293T cells were transfected with MyD88-flag plasmid for 24 h, and then treated with graded concentration (20 or 40 μmol/L) of soyasaponin (A₁, A₂ or I) or ST2825 (a MyD88 inhibitor) for 6 h. The protein levels of TLR4 (a), MyD88 (b), TRAF6 (c), p-p65 and p65 (d) were measured by western blotting. Results reported are Means ± SD of three independent experiments. Data were statistically analyzed by using one-way ANOVA of SPSS software. *: p < 0.05 v.s. control, #: p < 0.05 v.s. MyD88-flag plasmid transfected group