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Fig. 6 | BMC Complementary Medicine and Therapies

Fig. 6

From: Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells

Fig. 6

The combined effects of CUR, DMC, and BDMC on cell viability (a), apoptosis (b), and colony formation (d) in HOS cells. HOS cells treated with 10 μM CUR, DMC, and BDMC or their combination as indicated in the figure for 24 h were detected using MTT assay, annexin V-FITC /7-AAD staining, and clonogenic assay. c Quantitative analysis of the percentage of early apoptotic and late apoptotic cells in HOS cells. The percentage of cell viability was calculated as the ratio: 100% x (A540 absorbance of the drug-treated wells / (A540 absorbance of the no drug-treated wells). * Indicates the values are significantly different than the control (*P < 0.05, **P < 0.01, ***P < 0.001)

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