Fig. 9

Effects of CMC1, CMC7, and CMC10 on insoluble pS129-α-syn and aggregation. Insoluble pS129 and aggregation of α-syn were assessed in 10 μg and 15 μg of insoluble a and soluble b proteins from cell lysates of untransfected (control) and transfected HEK cells by immunoblotting proteins using antibodies specific to pS129-α-syn and total α-syn (Syn1). While one group of wt α-syn transfected HEK cells were simultaneously transfected with seeds and CMC at different molar ratios (1:5 and 1:20) after an incubation at 37 °C for 1 h, the other group was transfected with seeds to be followed by CMC treatment at different molar ratios (1:5 and 1:20) in OptiMEM for 48 h. Recombinant pS129-α-syn (rpS129-α-syn) and recombinant α-syn (r-α-syn) proteins were loaded (50 ng) as positive controls. Re-immunoblotting with β-actin antibody was performed to normalize the amount of loaded proteins