Fig. 6

Effect of CMC1 and CMC7 on the seeding of α-syn monomers with fibrils. α-syn monomers (100 μM) were seeded with 2 μM sonicated α-syn fibrils, which were then incubated in the presence or absence of a CMC1 and b CMC7 at different concentrations (10 and 50 μM) for 6 h with continuous shaking at 37 °C. The extent of fibril formation was estimated by Th-T binding assay. The assays were performed in triplicate (average of triplicate measurements ± standard deviations). c Electron microscopy images of negatively stained samples of α-syn incubated with seeds alone or in the presence of CMC1 or CMC7 in a concentration of 50 μM for the indicated times with continuous shaking at 37 °C. Scale bar, 500 nm. d Th-T binding assay for seeding the aggregation of α-syn monomers by either 2 μM of untreated seeds (wt seeds) or seeds generated from the incubation of CMC1 or CMC7 with α-syn fibrils for 5 days at a molar ratio of 4:1