Fig. 2

Effect of CMCs on α-syn fibril formation. a and b Fibril content formation analysis. α-syn protein (25 μM) was incubated for 5 days at 37 °C with continuous shaking in the absence (aged alone) or presence of CMC1, CMC7, CMC10, and CMC11 using different molar ratios (CMC: α-syn at 1:1, 2:1, and 4:1). Fibril content for each sample was then measured using a Thioflavin-T (Th-T) and b Congo red binding assays. Means ± standard deviations are from triplicates of one experiment. c Silver staining for SDS-PAGE of α-syn monomers, α-syn aged alone or in the presence of CMC1, CMC7, CMC10 and CMC11 at molar ratio 1:1 after Protein Kinase (PK) digestion. d Negative stain electron microscopy images showing fibril formation of α-syn aged alone or in the presence of the indicated CMCs (CMC: α-syn molar ratios of 1:1, 2:1, and 4:1). Scale bar, 500 nm. e Th-T binding assay for α-syn aged alone or with CMC1 or CMC7 for 5, 10 and 15 days at a molar ratio of 4:1 (CMC: α-syn). Means ± standard deviations are from the triplicates of one experiment. f Negative stain electron microscopy images showing fibril formation of α-syn aged alone or in the presence of CMC1 and CMC7 using molar ratio of 4:1 (CMC: α-syn) for 5, 10 and 15 days. Scale bar, 500 nm