Fig. 4

The effects of CF on NFATc1 and c-Fos expression. RAW 264.7 cells were treated with RANKL (100 ng/ml) and CF (1, 10, 100 μg/ml) for 4 days. A NFATc1 and c-Fos mRNA expression was detected using RT-PCR. a mRNA expression was normalized to GAPDH. B NFATc1 and c-Fos protein levels were determined by western blot. b Protein expression was normalized to GAPDH and actin. Data represent the means ± SEM of three independent experiments. ^##P < 0.01, ^#P < 0.05 compared with normal and ^**P < 0.01, ^*P < 0.05 compared with control. NFATc1, nuclear factor of activated T-cells; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Nor, nontreated; Con, RANKL-treated control