Fig. 3

The effects of CF on TRAF6, NF- κB and MAPK expression. RAW 264.7 cells were treated with RANKL (100 ng/ml) and CF (1, 10, 100 μg/ml) for 30 min. A TRAF6 and NF- κB expression were detected using western blot. a Protein expression was normalized to actin. B Phosphorylation of MAPKs were determined by western blot. b Protein expression was normalized to each total form. Data represent the means ± SEM of three independent experiments. ^##P < 0.01 compared with normal and ^**P < 0.01, ^*P < 0.05 compared with control. TRAF6, tumor necrosis factor receptor-associated factor 6; NF- κB, nuclear factor-kappa B; MAPKs, Mitogen-activated protein kinase. ERK, extracellular-signal-regulated kinase; JNK, c-Jun N-terminal kinases; Nor, nontreated; Con, RANKL-treated control