Fig. 2

The effects of CF on RANKL-induced osteoclastogenesis and bone resorptive activity in RAW 264.7 cells. a TRAP-positive cells were captured using an inverted microscope (100×). b TRAP-positive cells were counted, and c TRAP activity was measured by an ELISA reader. d Pit area was captured using an inverted microscope (100×). e Pit area was measured using ImageJ software. f The cell viability of RAW 264.7 cells in the presence of various concentrations of CF was determined by MTS assay. Data represent the means ± SEM of three independent experiments. ^##P < 0.01 compared with normal and ^**P < 0.01 compared with control. TRAP, tartrate-resistant acid phosphatase; RANKL, receptor activator of nuclear factor kappa β ligand