Fig. 2

GP prevents HFD-associated Non-alcoholic fatty liver disease (NAFLD) in the rat model. a, (b) Serum concentrations of ALT and AST in various groups compared with the NAFLD group (n = 10 for each group). Data are mean ± standard deviation. *P < 0.05; **P < 0.01 (one-way ANOVA with Newman–Keuls post hoc analysis). c H&E staining of the liver tissue in the NAFLD, Control, DLPC, GPL, GPM and GPH groups (magnification, 200×) (n = 10 for each group). Major histopathological changes induced by HFD in rat liver included hepatosteatosis, ballooning and hiver inflammation. d Liver lipid content was determined by Oil-red O staining in the NAFLD, Control, DLPC, GPL, GPM and GPH groups (magnification, 200×) (n = 10 for each group)