Fig. 2

Effects of DHYZ on H₂O₂-induced apoptosis. DHYZ and H₂O₂ treated PC12 cells were stained with Annexin V/PI, apoptosis was identified by flow cytometry (A) and expressed as mean ± standard deviation (the sum of early apoptosis (right lower quadrant) and late apoptosis (right upper quadrant)) (B). a, normal group; b, H₂O₂ group; c, control group; d, H₂O₂ + DHYZ (50 μg/ml) group; e, H₂O₂ + DHYZ (100 μg/ml) group; f, H₂O₂ + DHYZ (200 μg/ml) group. ^△P < 0.01, versus normal group; ^***P < 0.001, versus control group. The data are mean ± SD of three independent experiment each in triplicate