Skip to main content
Fig. 7 | BMC Complementary Medicine and Therapies

Fig. 7

From: The methanol extract of Guettarda speciosa Linn. Ameliorates acute lung injury in mice

Fig. 7

MGS suppresses NF-κB and neutrophil elastase activity. (a) RAW 264.7 cells were treated with MGS for 16 h and subsequently with 100 ng/mL of LPS for 15 min. Nuclear proteins were analyzed by immunoblotting for p65 RelA. The same blot was stripped and reblotted for lamin B. Similar experiments were performed three times. (b) MGS diluted in PBS was added to neutrophil elastase at final concentrations of 0.01, 0.1, 1, 10 μg/mL. Elastase activity was measured every minute for 10 min by ELISA assay per the instruction of the manufacturer. Data represent the mean ± SEM of three independent measurements. *P and **P were less than 0.01 and 0.001, respectively, compared to the control elastase activity

Back to article page
\