Fig. 6From: The methanol extract of Guettarda speciosa Linn. Ameliorates acute lung injury in miceMGS suppresses the ubiquitination of Nrf2. HEK 293 cells transfected with plasmids encoding V5-Nrf2, HA-Ub, and Keap1 were treated with MGS for 16 h. After V5-Nrf2 was immunoprecipitated by an anti-V5 antibody, the ubiquitinated V5-Nrf2 was analyzed by an anti-HA antibody. The cell lysate was analyzed by immunoblotting for V5-Nrf2 and GAPDH as for inputsBack to article page