Fig. 2

ROS production and cytotoxicity by MGS. (a) A flow cytometric analysis of intracellular ROS. After treated with LPS (100 ng/mL) or MGS (50 μg/mL and 100 μg/mL in PBS) for 16 h, RAW 264.7 cells were stained with carboxy-H₂DCFDA, a ROS indicator. One thousand cells were counted, and cells producing intracellular ROS are shown in percentile, compared with untreated control. (b) MTT assay for cell viability. Increasing amounts of MGS were added to RAW 264.7 cells. After 16 h, metabolically active cells were determined after measuring the formazan product by a spectrophotometer. Data represent the mean ± SEM of triplicate samples. No statistical differences between groups were found (ANOVA)