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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract

Fig. 4

The protein expression changes of MTDH and PTEN in SKOV3/DDP cells treated with the sera containing GFWE. (a) Representative images for MTDH and PTEN investigated. All images were obtained using a 40× Plan-Apochromat objective of the LSM800 confocal microscope. The nuclei were counterstained with DAPI (blue). 400× magnification, bar = 20 μm. (b) The MFIs of MTDH and PTEN in the cells measured with the graphics module of Zeiss Zen software. Error bars represent the SD. (c) Western blotting analyses demonstrated a strong decrease in the levels of MTDH protein and a strong increase in the levels of PTEN in SKOV3/DDP cells by the sera or LY294002. The mAb-PTEN, pAb-MTDH and mAb-GAPDH were used for blotting. A total of 20 μg of protein per sample was loaded to each lane. * P < 0.5, **P < 0.01. NS = No significant difference

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