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Fig. 1 | BMC Complementary Medicine and Therapies

Fig. 1

From: Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion

Fig. 1

Effects of OCP on cell viability, triglyceride content and glucose uptake in 3 T3-F442A differentiating adipocytes. 3 T3-F442A pre-adipocytes were grown for 10 days in culture medium renewed every 2–3 days. Opuntia cladode powders (OCP), O. streptacantha (OSC) and O. ficus-indica (OFI) were diluted directly in the culture medium (concentrations used were 1, 10, 100 μg/mL). a, b Cell viability was assessed in pre-adipocytes (left panel) and differentiated adipocytes (induced by 50 nM insulin; right panel) using the MTT assay after treatment with OSC (a); OFI (b). Graphs show the average values of three independent experiments. Results are expressed as mean percentage of the control (cells without OCP) in non-differentiated adipocytes. Statistical analyses involved ANOVA, followed by Newman–Keuls post hoc test. (c, d) TG content of 3 T3-F442A adipocytes was evaluated in pre-adipocytes (left panels) and differentiated adipocytes (right panels) treated with OSC (c); OFI(d). Data represent the mean percentage levels of the control (without OCP) in non-differentiated adipocytes normalised to protein content. Statistical analyses involved ANOVA, followed by Newman–Keuls post hoc test. **p < 0.01, ***p < 0.005 indicates significant difference from control without insulin and treatment; §§ p < 0.01 indicates significant difference between groups in insulin-treated cells. (e, f) Insulin-stimulated uptake of glucose in 3 T3-F442A adipocytes and effect of OCP. Glucose uptake was evaluated in pre-adipocytes (left panels) and differentiated adipocytes (right panels), with/without OCP treatment with OSC (c);OFI (d). Data are the mean percentage levels of the control (without OCP) in non-differentiated adipocytes normalised to protein content. Cytochalasin (cyto) 10 μM, was used as the negative control for glucose uptake. Statistical analyses involved ANOVA, followed by Newman–Keuls post hoc test. $p < 0.005 significantly different from all groups; **p < 0.01, *p < 0.05 significant difference from control without insulin and treatment; §§p < 0.01, §§§p < 0.005 indicates significant difference between groups in insulin-treated cells

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