Table 1 PCR conditions, amplicon size for each target gene, and primers used in this study
From: Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation
Gene | Primer sequences | Annealing temperature | Size (bp) |
|---|---|---|---|
ITGB1 | Forward: 5′-GTCGTGTGTGTGAGTGCAAC-3′ Reverse: 5′-GCTGGGGTAATTTGTCCCGA-3’ | 60 °C | 318 |
ITGB3 | Forward: 5’-CTGCCGTGACGAGATTGAGT-3′ Reverse: 5′-TGCCCCGGTACGTGATATTG-3’ | 60 °C | 383 |
ITGB5 | Forward: 5’-ACCTGGAACAACGGTGGAGA-3′ Reverse: 5′-AAAAGATGCCGTGTCCCCAA-3’ | 60 °C | 217 |
L-selectin | Forward: 5’-ATTTCCTGGCACATCATG-3′ Reverse: 5′-ATTGTCTCGGCAGAATCT-3’ | 56 °C | 95 |
LIF | Forward: 5’-ACAGAGCCTTTGCGTGAAAC-3′ Reverse: 5′-TGGTCCACACCAGCAGATAA-3’ | 56 °C | 78 |
E-cadherin | Forward: 5’-GGCCTGAAGTGACTCGTAACG-3′ Reverse: 5′-TCAGACTAGCAGCTTCGGAACC-3’ | 60 °C | 201 |
β-actin | Forward: 5’-CAAGAGATGGCCACGGCTGCT-3′ Reverse: 5′-TCCTTCTGCATCCTGTCGGCA-3’ | 56 or 60 °C | 275 |