Fig. 5
From: Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation
In vitro application and functional effects of D-Exo on implantation model. a Scanning electron microscope of exosomes from Ishikawa cells at magnification × 30, 000. b Results of western blot analysis for the exosome surface marker, CD9 and CD63, in FBS, V-Exo, D-Exo, and culture medium. c JAr cell invasion assay. D-Exo and V-Exo (control) were respectively treated with 2 μL at 5.0 × 105 cells/mL for 11 h. Photomicrographs on the left represent the cells that have passed through Matrigel. Original magnification × 200. The graph on the right shows relative cell invasion rate compared to the control. d Effects of D-Exo in the Ishikawa cells on adhesion of JAr spheroids. Data are expressed as mean ± SD. The experiments were repeated in triplicate wells. *P < 0.05 and **P < 0.01 are considered significant. D-Exo, exosomes isolated from decursinol-treated Ishikawa cells; V-Exo, exosomes isolated from vehicle-treated Ishikawa cells