Fig. 2

Real-time qPCR was performed to assess the effect of decursinol on the mRNA expression of ITGB1, ITGB3, ITGB5, L-selectin, LIF, and E-cadherin. a ITGB1, b ITGB3, c ITGB5, d L-selectin, e LIF, and f E-cadherin mRNA in the Ishikawa cells transfected at 12 h and 24 h following decursinol treatment (40 or 80 μM). Data shown are normalized mRNA fold change. Values are expressed as mean ± SD. The experiments were repeated in triplicate wells. *P < 0.05 and **P < 0.01 are considered significant