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Table 2 Antioxidant activity (as % and IC50) of the four promising algal fractions from the crude extract {(0.316 mg/L (Cu) of C.vulgaris compared to BHT and vitamin C as standards against DPPH (%) radical method

From: Evaluation of antioxidant and anticancer activity of crude extract and different fractions of Chlorella vulgaris axenic culture grown under various concentrations of copper ions

Fraction

100% Hexane

Hexane:Chloroform

(6:4)

Hexane:Chloroform

(5:5)

Hexane:Chloroform

(4:6)

BHT

Vit.C

Conc.

μg/ml.

(μg/ml)

 

30 min.

60 min.

30 min.

60 min.

30 min.

60 min.

30 min.

60 min.

30 min.

60 min.

30 min.

60 min.

500

95.1±12.0a

96.1±1.6a

94.0±2.0a

95.1±2.1a

97.2±2.1a

97.1±2.1a

93.7±2.0a

92.±1.9a

96.3±1.5a

97.4±3.0a

93.4±1.1a

94.5±2.8a

250

82±1.34b

80.5±1.8b

83.3±1.9b

82.6±1.9b

86±1.20b

85.7±1.9b

80±1.4b

81.±1.8b

92.7±1.0b

93±2.2b

90.3±1.0b

90.9±2.2b

125

77±1.08c

77.5±1.6c

74.5±1.7c

75.8±1.3c

84.0±81.5b

83.9±1.4c

78.5±2.0c

77.1±1.3c

90.3±0.9c

89.0±2.0c

88.8±0.9c

88.3±1.9c

62.50

60.7±1.11d

61.1±1.7d

63.3±1.6d

64.8±1.3d

79.5±1.02c

80.7±1.9d

71±1.0d

70.9±1.9d

84.4±0.4d

84.9±2d

83.7±1.1d

85.9±1.0d

31.25

59.3±0.9d

60.9±0.9d

56.3±1.1e

55.1±1.1e

74.4±1.2d

75.9±1.4e

60.3±0.9e

61.9±1.2e

72.7±0.5e

73.2±1.9e

68.2±0.7e

69.5±0.9e

15.62

57.5±0.8e

56.9±0.7e

53.5±1.2f

54.0±0.9e

65.8±1.3e

67.09±1.f

59.3±0.7e

60.4±1.1e

70.2±0.8f

69.4±1.2f

64.4±0.5f

63.0±0.9f

IC50

13.58

13.72

14.59

14.46

11.87

11.65

13.18

12.93

11.1

11.2

12.1

12.9

  1. Different small letters on the column for each fraction indicate significant difference (p< 0.05). Error bars represent ±SD of three replicates