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Table 1 Primers used in real-time qPCR

From: Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells

Gene Primer sequences Product (bp)
HIF-1α forward: 5′-CCAGATTCAAGATCAGCCAGCA-3′
reverse: 5′-GCTGTCCACATCAAAGCGTATA-3´
100
VEGF forward:5´-ACATTGGCTCACTTCCAGAAACA-3′
reverse:5-TGGTTGGAACCGGATCTTTA-3´
108
Bcl-2 forward:5′-GGTGGTGGAGAACTCTTCACGT-3′
reverse:5´-AGGATTGTGGCTGAACA-3´
253
Bax forward: 5´-TGGCGATGAACTGGACAACAA-3′
reverse: 5′-GGGAGTCTGTATCCACATCAGCA-3´
65
Caspase-3 forward:5´-AGACAGACAGTGGAACTGACGATG-3′
reverse:5′-GGCGCAAAGTGACTGGATGA-3´
147
Cytochrome C forward:5-GAAGAAGGGAGAAAGGGCAGA-3′
reverse:5′-CGGGGGCTGTCCAACAAA-3´
302
GAPDH forward: 5´-GCCACAGTCAAGGCTGAGAATG-3′
reverse:5′-ATGGTGGTGAAGACGCCAGTA-3´
143