Fig. 4

Rhy inhibits the JAK2/STAT3 signaling pathway. a-c After treatment with Rhy, the levels of JAK2, p-JAK2, STAT3, p-STAT3 and nuclear STAT3 were detected by western blot. d Level of STAT3 in the bronchus was elevated by immunofluorescence. Bar = 50 μm; White arrows indicate nuclear STAT3. Red fluorescence, STAT3; blue fluorescence, DAPI. N = 6 for each group. The results were presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Rhy, rhynchophylline; JAK2, janus kinase 2; STAT3, signal transducer and activator of transcription 3; SD, standard deviation