Skip to main content
Fig. 5 | BMC Complementary Medicine and Therapies

Fig. 5

From: Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells

Fig. 5

Intracellular ROS, AKT/mTOR and Erk signaling pathways were associated with the cytotoxicity induced by QIG. a-b Cells were treated with QIG in combination with or without NAC (2.5 mM, 5 mM and 10 mM) for 24 h. The level of intracellular ROS was assessed by fluorescence microplate reader after cells were treated with ROS assay kit (*P < 0.05, ***P < 0.001). c-d Cell viability was measured by CCK8 assay (**P < 0.01, ***P < 0.001). e Western blotting was used to detect the expression of protein cleaved-PARP and β-Tubulin. f-g HT-29 cells were dose dependently treated with QIG, then the protein levels of p-mTOR-S2448, p-AKT-S473, P-P70S6K-S371, p-4EBP1-pT45, Erk1/2, p-Erk1/2-T202/Y204, β-Tubulin and β-actin were assessed by western blotting and quantified by Image J software. h Cells were treated with QIG (0.3 mg/ml) in combination with or without 2.5 mM NAC for 24 h and the expression of proteins p-mTOR-S2448, p-AKT-S473, p-P70S6K-S371, p-4EBP1-pT45 and β-actin were analyzed by western blotting and quantified by Image J software

Back to article page