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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells

Fig. 4

Inhibition of autophagy attenuated QIG-induced cytotoxicity in HT-29 cells. a-b Autophagy marker protein LC3-I/II were assessed by western blotting in HT-29 cells after treated with 0.3 mg/ml QIG in combination with or without 5 μM LY394002 or 5 nM BAF-1. c-d CCK8 assay was used to detect the cell viability (*P < 0.05, ***P < 0.001). e After cells were treated with QIG (0.3 mg/ml) and siATG5 for 24 h, the expression of proteins LC3-I/II and cleaved-PARP, cleave-caspase 3 were analyzed by western blotting and quantified by Image J software. f CCK8 assay was used to detect the cell viability (**P < 0.01)

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