Fig. 5

PKC-δ silencing inhibits FFA-induced L02 hepatocyte steatosis. Control siRNA group (NC), control siRNA + FFA group (NF), control siRNA + FFA + 10% high dose of HQT-medicated serum group (NH), PKC-δ siRNA group (SC), PKC-δ siRNA + FFA group (SF) and PKC-δ siRNA + FFA + 10% high dose of HQT-medicated serum group (SH). a Oil Red O staining (original magnification: × 400). b Lipid content in the cells determined by Oil red-based colorimetric assay. c TG (triglyceride) content. d and e The expression levels of SREBP-1C and FOXO1 protein in L02 cells with or without transfection of PKC-δ siRNA. Results are expressed as mean ± S.D. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs NC group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs NF group; ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001, vs SC group; ^&* p < 0.05, ^&&* p < 0.01, ^&&* p < 0.01 vs SF group