Fig. 4

PKC-δ silencing recovers Calcium homeostasis in ER. control siRNA group (NC), control siRNA + FFA group (NF), control siRNA + FFA + 10% high dose of HQT-medicated serum group (NH), PKC-δ siRNA group (SC), PKC-δ siRNA + FFA group (SF) and PKC-δ siRNA + FFA + 10% high dose of HQT-medicated serum group (SH). a The changes of calcium fluorescence densities were monitored in Fluo-4/AM-loaded L02 cells. b Effects of HQT-medicated serum on SERCA activity induced by FFA in normal L02 cells and PKC-δ knockdown L02 cells. c and d The expression levels of SERCA2, CANX, and IP3R protein in L02 cells with or without transfection of PKC-δ siRNA. Results are expressed as mean ± S.D. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs NC group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs NF group; ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001, vs SC group; ^&* p < 0.05, ^&&* p < 0.01, ^&&* p < 0.01 vs SF group