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Fig. 1 | BMC Complementary Medicine and Therapies

Fig. 1

From: Hugan Qingzhi medication ameliorates free fatty acid-induced L02 hepatocyte endoplasmic reticulum stress by regulating the activation of PKC-δ

Fig. 1

HQT-medicated serum reduces FFA-induced L02 hepatocyte steatosis. a and b LDH release assay for each medicated serum treatment in FFA-induced L02 cells. L02 cells were treated with or without different concentrations of blank vehicle serum (0,10,20,30,40%) for 24 h (a); L02 cells were treated with HQT-L, HQT-M, HQT-H, FF and vehicle serum, respectively (b). c Lipid droplets were observed by Oil Red O staining (original magnification: × 400). d Lipid content in the cells determined by Oil red-based colorimetric assay. e TG content in FFA-induced L02 hepatocytes. f AST and ALT levels in FFA-induced L02 hepatocytes. Results are expressed as means ± S.D. ***p < 0.001 compared with the 10% FBS group. **p < 0.01, ***p < 0.001 vs CON group, #p < 0.05, ##p < 0.01, ###p < 0.001 vs FFA group

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